Viable but Nonculturable(VBNC) State of Pathogenic Bacteria.

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The viable but nonculturable state in bacteria.

It had long been assumed that a bacterial cell was dead when it was no longer able to grow on routine culture media. We now know that this assumption is simplistic, and that there are many situations where a cell loses culturability but remains viable and potentially able to regrow. This mini-review defines what the "viable but nonculturable" (VBNC) state is, and illustrates the methods that ca...

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Viable but Non-culturable Bacteria: Clinical Practice and Future Perspective

In this editorial, the importance of VBNC in clinical practice and future researches interests has been pinpointed. Accurate detection of VBNC, especially for major mentioned pathogens, is the future research gap. 

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Current Perspectives on Viable but Non-Culturable (VBNC) Pathogenic Bacteria

Under stress conditions, many species of bacteria enter into starvation mode of metabolism or a physiologically viable but non-culturable (VBNC) state. Several human pathogenic bacteria have been reported to enter into the VBNC state under these conditions. The pathogenic VBNC bacteria cannot be grown using conventional culture media, although they continue to retain their viability and express...

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Persistence and Potential Viable but Non-culturable State of Pathogenic Bacteria during Storage of Digestates from Agricultural Biogas Plants

Despite the development of on-farm anaerobic digestion as a process for making profitable use of animal by-products, factors leading to the inactivation of pathogenic bacteria during storage of digestates remain poorly described. Here, a microcosm approach was used to evaluate the persistence of three pathogenic bacteria (Salmonella enterica Derby, Campylobacter coli and Listeria monocytogenes)...

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Detection of Viable But Non-Culturable State of Escherichia coli O157:H7 Using Reverse Transcription PCR

Background and Aims: Many bacteria including Escherichia coli may enter into a viable but non-culturable (VBNC) state under unfavorable stresses, which are unable to be detected by culture-based methods. In this study, the use of Reverse Transcription PCR (RT-PCR) for detection of VBNC state of E. coli O157:H7 was investigated. Materials and Methods:  Escherichia. coli O157:H7 was inoculated i...

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ژورنال

عنوان ژورنال: Microbes and Environments

سال: 1997

ISSN: 1342-6311,1347-4405

DOI: 10.1264/jsme2.12.135